Chemical Products and Consumables, PCR Primers Sets

• Country: Lebanon
• Summary: Chemical Products and Consumables, PCR Primers Sets
• LBT Ref No: 70238341
• Deadline: 18 Aug 2022
• Financier: Self Financed
• Purchaser Ownership: Government
• Tender Value: Refer Document
• Notice Type: Tender
• Document Ref. No.:
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• Description:
• Tenders are invited for Chemical Products and Consumables, PCR Primers Sets Kits for Fish Bacterial Isolation. 1- Chemical products and consumables Items Special Features Category Qtty 1 Petri dishes 9cm Box 2 2 Petri dishes 5cm Box 1 3 Bacteriological PE Loop Box 1 4 PCR tubes 0.2 mL Box 1 5 Ethanol 96 20L 1 6 UV stain loading dye 6X 1U 1 7 Trizma HCl 1Kg 1 8 Agarose puriss DNA electrophoresis 100g 1 9 Red Taq Ready PCR Mix 10XRN PCR Mix 1x2.5 mL 2 10 1 Kb DNA Ladder Direct Load Ready to load 500uL 1 11 TAE 10X 1L 2 12 Proteinase K ≥ 30 U/mL From T. album 10mg 2 13 Tetrathionate broth 500g 1 14 TCBS agar 500g 1 15 Bile salts Irgasan Brilliant green agar 500g 1 16 Ampicillin 5g 1 2- PCR Primers Sets Item Primer Unit Qtty Tenacibaculum maritimum 16S rRNA MAR1 AATGGCATCGTTTTAAA 16S rRNA MAR2 CGCTCTCTGTTGCCAGA Set of primers 1 Flavobacterium psychrophilum 20F 8-27 AGA GTT TGA TCA TGG CTC AG 1500R 1510-1492 GGT TAC CTT GTT ACG ACT T PSY1 190-206 GTT GGC ATC AAC ACA CT PSY2 1278-1262 CGA TCC TAC TTG CGT AG Set of primers Set of primers 1 1 Acinetobacter johnsonii/lwoffii/baumannii U8f 5- AGAGT TTGATCMTGGCTCAG 3- U1492 5- TACGGY TACCTTGTTACGACTT 3- Set of primers 1 E. rhusiopathiae ER1F 59-GTTCATCTCTCTAATGCACTAC- ER1R 59-TGTTGGACTACTAATCGTTTCG- Set of primers 1 Pleisomonas shigelloides 5′-GCG AGC GGG AAGGGA AGA ACC-3- 5′-GTC GCC CCA AAC GCT AAC TCA TCA-3′ Set of primers 1 Aeromonas sp. AP1 (5‘-GGCTGATCTCTTCATCCTCACCC-3-) AP2 (5‘-CAGAGTGAAATCTACCAGCGGTGC-3-), MIY1 (5‘-AGCCTCCACGCGCTCACAGC-3-) MIY2 (5‘-AAGAGGCCCCATAGTGTGGG-3-), Set of primers Set of primers 1 1 Pseudomonas baetica/plecoglossicida PA-GS-F (5′-GACGGGTGAGTAATGCCTA-3′) PA-GS-R (5′-CACTGGTGTTCCTTCCTATA-3′) PL-G1F CACAGGCTCCAATGGCTGTA PL-G1R AGCCCAGTGCAGTGATCAGC PL-G2F TGCTGAAGGACGAGCGTTCG PL-G2R ATCATCTTGCCGACAACAGC Set of primers Set of primers Set of primers 1 1 1 Renibacterium salmoninarum DN1 F5′-GAAAATAAAAAAAATTTTAGCGCTG-3′ DN2 R 5′-CTTGGCAGGACCATCTTTGT-3′ Set of primers 1 Edwardsiella Sp. gyrBF1, 5￠-GCATGGAGACCTTCAGCAAT-3￠; gyrBR1, 5￠-GCGGAGATTTTGCTCTTCTT-3￠. Set of primers 1 Streptococcus inae A1 5¢-AG TCGTAACAAGGTAAGCCG-3¢ B15¢ CT/CA/GT/CTGCCAAGCATCCAC-T3¢. 5¢-GAAA GAAAATAGGAAAGAGACGCAGTGTC-3¢ SP-2 5¢-CCTTATTTCCAGTCTTTCGACCTTC-3¢ Set of primers Set of primers 1 1 Vibrio sp. Vp-tlh1 GATTTGGCGAACGAGAAC V. parahaemolyticus Vp-tlh2 CGTCTCGAACAAGGCG VctoxR403F GAAGCTGCTCATGACATC V. cholerae VctoxR678R AAGATCAGGGTGGTTATTC WhA870F ACTCAACTATCGTGCACG V. vulnificus WhA1236R ACACTGTTCGACTGTGAG Set of primers Set of primers Set of primers 1 1 1 Photobacterium piscicida CPS F AGGGGATCCGATTATTACTG CPS R TCCCATTGAGAAGATTTGAT Set of primers 1
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